Derivation of Aromatic Amino Acid Mutants from a Methanol-Utilizing Yeast, Hansenula polymorpha
نویسندگان
چکیده
منابع مشابه
Selective enrichment of aromatic amino acid auxotrophs in Hansenula polymorpha.
Aromatic amino acid auxotrophs of the methanol-utilizing yeast Hansenula polymorpha were effectively selected by the use of nystatin and a medium that inhibits the growth of tyrosine auxotrophs. The procedure resulted in a frequency of aromatic auxotrophs of 2% of survivors and an enrichment of 20-fold. The new procedure also takes less time than traditional procedures. Of the auxotrophic mutan...
متن کاملMethanol oxidation and assimilation in Hansenula polymorpha
The metabolism of methanol was monitored in whole cells of the methylotrophic yeast Hansenula polymorpha by using [13C]methanol and n.m.r. in vivo. The main products observed under normal conditions were trehalose and glycerol, whereas cells that were starved before exposure to [13C]methanol also accumulated glutamate, glutamine and alanine; formate was also more prominent in spectra from starv...
متن کاملCreation of Hansenula polymorpha fatty acid auxotrophic mutants for genetic study of fatty acid biosynthesis
Three groups of Hansenula polymorpha fatty acid auxotrophic mutants were screened by their fatty acid requirement for growth. The mutants are saturated fatty acid auxotrophic mutants (Sfa), monounsaturated fatty acid auxotrophic mutants (Mfa) and polyunsaturated fatty acid auxotrophic mutants (Pfa) which required saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids...
متن کاملHARO7 encodes chorismate mutase of the methylotrophic yeast Hansenula polymorpha and is derepressed upon methanol utilization.
The HARO7 gene of the methylotrophic, thermotolerant yeast Hansenula polymorpha was cloned by functional complementation. HARO7 encodes a monofunctional 280-amino-acid protein with chorismate mutase (EC 5.4. 99.5) activity that catalyzes the conversion of chorismate to prephenate, a key step in the biosynthesis of aromatic amino acids. The HARO7 gene product shows strong similarities to primary...
متن کاملHansenula polymorpha Swi1p and Snf2p are essential for methanol utilisation.
We have cloned the Hansenula polymorpha SWI1 and SNF2 genes by functional complementation of mutants that are defective in methanol utilisation. These genes encode proteins similar to Saccharomyces cerevisiae Swi1p and Snf2p, which are subunits of the SWI/SNF complex. This complex belongs to the family of nucleosome-remodeling complexes that play a role in transcriptional control of gene expres...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1981
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.41.5.1088-1096.1981